Eukaryotic Translation Initiation Factor 2B, epsilon, phosphorylated (Ser539) (eIF2Be)

Eukaryotic Initiation Factor 2B (eIF2B) plays a key role in the initiation of mRNA translation in eukaryotic cells by acting as a guanine nucleotide exchange factor. The activity of eIF2B is regulated by a wide array of conditions. Stress inhibits its action, while stimulation of protein synthesis results in its activation. eIF2BE, an 82kD subunit of eIF2B, is phosphorylated at serine 539 by glycogen synthase kinase-3 (GSK-3), which leads to inhibition of its guanine nucleotide exchange activity.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Positive Controls: \nRecombinant eIF2BE treated with GSK-3b; CHO-T cells transfected with human insulin receptor (IR) +/- insulin.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

E8949-21B

Size

10 Blots

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Rat

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by affinity chromatography.

References

1. Vary, T.C., et al. (2002) Am. J. Physiol. Endocrinol. Metab. 283(5):E1032-E1039. 2. Wang, X., et al. (2001) EMBO J. 20(16):4349-4359. 3. Woods, Y.L., et al. (2001) Biochem. J. 355(Pt 3):609-615. 4. Kleijn, M., and C.G. Proud (2000) FEBS Lett. 476(3):262-265. 5. Jefferson, L.S., et al. (1999) Int. J. Biochem. Cell. Biol. 31(1):191-200. 6. Kimball, S.R., et al. (1998) J. Biol. Chem. 273(5):3039-3044. 7. Welsh, G.I., et al. (1996) J. Biol. Chem. 271(19):11410-11413. 8. Price, N., and C. Proud (1994) Biochimie 76(8):748-760.

Additional Info

Recognizes rat eIF2BE when phosphorylated at Ser539. Species sequence homology: human (80%).