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Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1 (EIF4ENIF1, 4E-T, Clast4, eIF4E Transporter, FLJ21601)

Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1 (EIF4ENIF1, 4E-T, Clast4, eIF4E Transporter, FLJ21601)

Cat no: E8949-45I

Supplier: United States Biological
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eIF4E is a protein that modulates the translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. . Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:32000 Western Blot: 0.25-0.5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: E8949-45I
Reactivities: Human, Mouse, Rat
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris-saline, 0.5 mg/ml, 0.02% sodium azide, pH 7.3, 0.5% BSA.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
References: 1. Dostie J, Ferraiuolo M, Pause A, Adam SA, Sonenberg N. A novel shuttling protein, 4E-T, mediates the nuclear import of the mRNA 5' cap-binding protein, eIF4E. EMBO J. 2000 Jun 15;19(12):3142-56.
Additional info: Recognizes human 4E-T at ~140-150kD in 293 lysates (predicted size of ~108kD according to NP_062817.1). Species Crossreactivity: Mouse and rat.

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