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Eukaryotic Translation Initiation Factor 4E, phosphorylated (Ser209) (eIF4E, eIF4EL1)

Eukaryotic Translation Initiation Factor 4E, phosphorylated (Ser209) (eIF4E, eIF4EL1)

Cat no: E8949-40F

Supplier: United States Biological
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eIF4E is a eukaryotic translation initiation factor that is involved in the direction of ribosomes to the cap structure of mRNA's. There are two forms of eIF4E: a free form (25kD) and as part of a multi-protein complex (eIF-4F). The free form seems to not be as plentiful, compared to the other initiation factors and acts as a rate limiting step of initiation. Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry (paraffin), and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:5000-10000 Immunohistochemistry (paraffin): 1:100-250 Immunocytochemistry: 1:500 Immunoprecipitation: 1:60 Optimal dilutions to be determined by the researcher. Positive Control: 293 cell lysate Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: E8949-40F
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
P type: Mab
Isotype: IgG
Purity: Supernatant
References: 1. Rychlik, W., et al. (1987). Proc. Nat. Acad. Sci. 84: 945-949. 2. Rhoads, R. E., et al. (1993). Prog. Nucleic Acids Res. Mol. Biol.46:183-219. 3. Scheper, GC., et al. (2001). Molec. Cell. Biol.;743-754. 4. Jones, R., et al. (1996). Molec. Cell. Biol. 16: 4754-4764. 5. Wendel, H., et al. (2004). Nature 428: 332-337.
Additional info: Recognizes human eIF4E phosphorylated at Ser209. Species Crossreactivity: mouse, rat.

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