Exonuclease I (Exonuclease-I, ExoI, Exonuclease 1, Exonuclease-1, Exo1, HEX1, hExo1, hExoI, Rad2 Nuclease Family Member Homolog of S. cerevisiae Exonuclease 1)

This gene encodes a protein with 5' to 3' exonuclease activity as well as an RNase H activity. It is similar to the Saccharomyces cerevisiae protein Exo1 which interacts with Msh2 and which is involved in mismatch repair and recombination. Alternative splicing of this gene results in three transcript variants encoding two different isoforms. [provided by RefSeq]\n \nEpitope: C-HRNYSPRPESGT\n \nTheoretical Molecular Weight: 89.1kD\n \nApplications:\nSuitable for use in Peptide ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nPeptide ELISA: Antibody detection limit dilution 1:64,000\nWestern Blot: 0.2-0.5ug/ml; Approx 90kDa band observed in lysates in cell lines HEK293 and A549 (calculated MW of 89.1kDa according to NP_003677.3).\nOptimal dilutions to be determined by the researcher. \n \nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E9007-85D

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in Tris-saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Kim JC, Roh SA, Yoon YS, et al. MLH3 and EXO1 alterations in familial colorectal cancer patients not fulfilling Amsterdam criteria. Cancer Genet Cytogenet. 2007 Jul 15;176(2):172-4. No abstract available. PMID: 17656264

Additional Info

Recognizes human Exonuclease 1. Expected to recognize all three reported isoforms (NP_003677.3; NP_006018.3 and NP_569082.1).