F4/80, Pan Macrophages (Cell Surface Glycoprotein EMR1, Cell Surface Glycoprotein F4/80, DD7A5-7, EGF-like Module-containing Mucin-like Hormone Receptor-like 1 Precursor, EGF-TM7, EMR1 Hormone Receptor, F4/80, Gpf480, Ly71, TM7LN3) (Biotin)

F4/80 is a 125kD extracellular macrophage membrane molecule, highly restricted to mature macrophage subpopulations residing in tissue. The antigen is detected on tissue fixed macrophages in all organs tested so far (spleen, lymph nodes, thymus, liver, skin). It is also present on Langerhans cells in the skin and Kupffer cells in the liver. It is absent on granulocytes, lymphocytes and trombocytes. The expression of F4/80 increases upon maturation of macrophage precursors in bone marrow and blood as well as in ontogeny. F0001-51A is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas. Furthermore it is a unique histological marker of the progression from peri-insulitis to beta-cell destruction and diabetes in a mouse diabetes model. Applications: Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50, Fixed with 1% paraformaldehyde (1) Western Blot: 1:50, Mouse bone marrow-derived macrophages; non-reduced; ~125kD; reduction with 2-mercaptoethanol destroys antigen. Immunohistochemistry (Frozen): 1:50, Tissues embedded in OCT Tissue Tec; fixed with acetone for 10 min at RT; incubation with 0.02M sodium azide in PBS, 0.1% H2O2 for 10 min at RT to destroy endogenous peroxidase. Immunohistochemistry (Paraffin): 1:50, Fixation in 10% neutral buffered formalin for 24 hours; blocking with non- immunized goat serum; microwaved for 6 min in citrate buffer. Optimal dilutions to be determined by the researcher. Controls: Positive: Mouse macrophages; IHC (F): Spleen; IHC (P): Splenic macrophages Negative: Mouse fibroblasts or granulocytes Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.