F4/80, Pan Macrophages (Cell Surface Glycoprotein EMR1, Cell Surface Glycoprotein F4/80, DD7A5-7, EGF-like Module-containing Mucin-like Hormone Receptor-like 1 Precursor, EGF-TM7, EMR1 Hormone Receptor, F4/80, Gpf480, Ly71, TM7LN3) (PE)

Murine F4/80 glycoprotein contains seven-transmembrane (TM7) regions, which anchor the protein in the cell membrane, and thereby shows similarity in this region to G-protein-coupled receptors. The F4/80 molecule shares overall structural homology to other members of the epidermal growth factor (EGF)-TM7 family. The antigen is detected on tissue fixed macrophages in all organs tested so far (spleen, lymph nodes, thymus, liver, skin). It is also present on Langerhans cells in the skin and Kupffer cells in the liver. It is absent on granulocytes, lymphocytes and thrombocytes. The expression of F4/80 increases upon maturation of macrophage precursors in bone marrow and blood as well as in ontogeny. This monoclonal antibody is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas. Furthermore it is a unique histological marker of the progression from peri-insulitis to beta-cell destruction and diabetes in a mouse diabetes model.\n\nApplications:\nSuitable for use in Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 10ul/250,000 cells in a 100ul total staining volume\nImmunohistochemistry (Frozen): tissue embedded in OCT Tissue Tec; fixed with acetone for 10min at RT; incubation with 0.02M sodium azide in PBS containing 0.1% H2O2 for 10 min at RT to destroy endogenous peroxidase; spleen as positive control \nImmunohistochemistry (Paraffin): fixation in 10% neutral buffered formalin for 24h; blocking with non-immunized goat serum; microwaved for 6min in citrate buffer; splenic macrophages as positive control\nWestern Blot: Mouse bone-marrow derived macrophages; non-reduced; ~125kD; reduction with 2-mercaptoethanol destroys BM8 antigen\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: Mouse macrophages\n\nStorage and Stability:\nMay be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4 degrees C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

F0001-51D

Size

100Tests

Applications

FC, IHC, WB

Hosts

Rat

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, 1% BSA, 0.02% sodium azide. Labeled with R-Phycoerythrin (PE).

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Malorny, U et al; A monoclonal antibody against an antigen present on mouse macrophages and absent from monocytes. Cell Tissue Res 1986, 243: 421. 2. Leenen, P et al; Markers of mouse macrophage development detected by monoclonal antibodies. J Immunol Methods 1994, 174: 5. 3. Mackler, A et al; Macrophage trafficking in the uterus and cervix precedes parturition in the mouse. Biol Reprod 1999, 61: 879. 4. Schaller, E et al; Inactivation of the F4/80 glycoprotein in the mouse germ line. Mol Cell Biol 2002, 22: 8035.

Additional Info

Recognizes mouse F4/80, a 125kD extracellular macrophage membrane molecule, highly restricted to mature macrophage subpopulations residing in tissue. Does not react with mouse granulocytes, mast cells, platelets, lymphocytes fibroblasts and endothelial cells. Species Crossreactivity: human (heart macrophages).