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Factor H (Complement Factor H, Beta 1H)

Cat no: F0010-01B

Factor H (Complement Factor H, Beta 1H)

Human complement factor H exists in 2 forms. The most common form, of 150kD, and the less common form of 43kD. Factor H is secreted by the liver into the blood serum. It is important in regulating the complement pathway, preventing unnecessary inflammation which can damage the host tissue. Complement factor H functions as a cofactor in the inactivation of C3b by factor I. It makes C3b susceptible to cleavage by factor I, resulting in iC3b. Factor H also inhibits the formation of the C3bBb complex (C3 convertase) and increases the rate of dissociation of both C3 convertase and the (C3b)NBB complex (C5 convertase). This prevents these components of the classical and of alternative complement pathways from forming a positive feedback loop. Mutations in factor H are associated with hemolytic-uremic syndrome, age-related macular degeneration, membranoproliferative glomerulonephritis (MPGN) type II and chronic hypocomplementemic nephropathy.\n\nApplications: \nSuitable for use in ELISA, Immunohistochemistry, Western Blot and Flow Cytometry. Other applications not tested.\n\nRecommended Dilutions:\nImmunohistochemistry: Frozen sections\nOptimal dilutions to be determined by the researcher.\n\nImmunohistochemistry Positive Control:\nKidney from post streptococcal glomerulonephritis patients.\n\nRecommended Secondary Antibodies:\nI1904-75E: IgG1 (HRP) Pab RbxMo\nI1904-79M: IgG1 (HRP) Pab GtxMo\nI1904-75D: IgG1 (FITC) Pab RbxMo\nI1904-76L: IgG1 (FITC) Pab GtxMo\n\nRecommended Negative Control:\nI1904-78R: IgG1 Murine Negative Control\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

F0010-01B

Size

100ug

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in BBS, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1

References

1. Alexander, J., et al., Mol. Immunol. 44: 123-132 (2007). 2. Harrison, R., et al., Mol. Immunol. 17: 9-20 (1980).

Additional Info

Recognizes human Complement Factor H at ~150kD or ~43kD.

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