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Factor H (Complement Factor H, Protein beta-1-H, Cfh, Hf1)

Cat no: F0010-01Q

Factor H (Complement Factor H, Protein beta-1-H, Cfh, Hf1)

Complement Factor H is a 155kD glycoprotein that negatively regulates the alternative pathway complement cascade. It is secreted by Kupffer cells, hepatocytes, vascular endothelial cells, and platelets and circulates in the serum at high concentration. CFH interacts with cell surface polyanions and prevents local complement activation by sequestering complement component C3b, accelerating the decay of C3 and C5, and functioning as a cofactor for Factor I. CFH is composed of 20 SCR. Alternate splicing generates an isoform that is truncated following SCR7. Within SCR15-20 aa857-1234, which encompass the primary binding sites for heparin and C3b, human CFH shares 60% and 63% aa sequence identity with mouse and rat CFH.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F0010-01Q

Size

100ug

Applications

WB

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a lyophilized powder in PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.

P Type

Mab

Purity

Purified by Protein A/G affinity chromatography.

Isotype

IgG2A

Additional Info

Recognizes mouse Complement Factor H. Does not react with rhComplement Factor H aa1-866, rhCD35, or recombinant mouse HABP1.

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
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