Factor VII (F.VII, Stable Factor, Proconvertin)

Coagulation factor VII is synthesized in the liver and circulates in the plasma where it binds to tissue factor (TF) and is rapidly converted into VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and also has important coagulation-independent functions such as angiogenesis. The predominant splicing variant of Factor VII in normal liver corresponds to the 444 amino acid precursor. After cleavage of a signal peptide (residues 1 to 38), the mature chain can be further processed into the light chain (residues 39 to 190) and the heavy chain (residues 191 to 444).\n\nApplications: \nSuitable for ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: Recombinant\nWestern Blot: Recombinant Factor VII or Factor VIIa and native Factor VII (Non-reduced)\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F0015-01

Size

100ug

Applications

ELISA, WB

Hosts

Rat

Reactivities

Rat

Form

Supplied as a liquid in 50% glycerol, H2O.

P Type

Mab

Purity

Purified by gel filtration and ion exchange chromatography.

Isotype

IgG1

Additional Info

Recognizes mouse Factor VII. Also recognizes VIIa (non-reduced Western Blot only). Species crossreactivity: Does not crossreact with human Factor VII or Factor VIIa.