Factor VIIa, Human

Participation of Factor VIIa in the Extrinsic Factor Xase Complex: Factor VIIa and its cofactor tissue factor (TF) assemble on a negatively charged membrane surface in a calcium dependent manner to form an enzyme complex which proteolytically converts factor X to factor Xa. The complex is referred to as the extrinsic factor Xase complex, because the tissue factor component is presumably derived from a source "extrinsic" to the plasma environment, which is accessible following vascular injury. \n

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SPECIFICATIONS

Catalog Number

F0016-14

Size

20ug

Form

Supplied as a liquid in 50% glycerol, H2O

Purity

(same/more than)95%. Purified by immunoaffinity chromotography of Factor VII. Followed by by Ion Exchange chromatography. Purified Factor VII is activated to Factor VIIa. The purity is determined by SDS-PAGE analysis.

References

1. Broze, G.J. and Majerus, P.W., Methods Enzymol., 80, 228 (1981).\n2. Bajaj, S.P., et al., J. Biol. Chem., 256, 253 (1981).\n3. Williams, E.B., et al., J. Biol. Chem., 264, 7536 (1989).\n4. Kisiel, W. and McMullen, B.A., Thrombosis Res., 22, 375 (1981).\n5. Radcliffe, R. and Nemerson, Y., J. Biol. Chem., 250, 388 (1975).\n6. Lawson, J.H.,et al., J. Biol. Chem., 268, 767 (1993).\n7. Kisiel, W., et al., Biochemistry, 16, 4189 (1977).\n8. Radcliffe, R., et al., Blood, 50, 611 (1977).\n9. Seligsohn, U., et al., J. Clin. Invest., 64, 1056 (1979).\n10. Kisiel, W., et al., Biochemistry, 14, 4928 (1975).\n11. McMullen, B.A., et al., BBRC, 115, 8 (1983).\n12. Discipio, R.G., et al., Biochemistry, 16, 698 (1977).