Factor VIII Related Antigen (vWF, von Willebrand Factor)

Human von Willebrand factor (factor VIII R:Ag) is a 270kD multimeric plasma gylcoprotein. It mediates platelet adhesion to injured vessel walls and serves as a carrier and stabilizer for coagulation factor VIII. The von Willebrand factor has functional binding domains to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. The factor is synthesized by endothelial cells and is also present in platelets and megakaryocytes.\n\nApplications: \nSuitable for use in Immunofluorescence and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (Paraffin): 1:200-1:400 using indirect Immunoflourescence. Enzymatically pre-digested with 0.1% pronase, formalin fixed. Specifically stains endothelial cells.\nImmunohistochemistry (Paraffin): 1:1000 using indirect immunoperoxidase. Enzymatically pre-digested with 0.1% pronase, formalin fixed and developed with Biotin/Avidin. Specifically stains blood vessels. \nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nFormalin-fixed, paraffin sections of human tongue\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

F0016-13C

Size

500ul

Applications

IF, IHC

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.

P Type

Pab

Purity

Purified by Ion Exchange chromatography.

Isotype

IgG

References

Bukh A et al. The multimeric structure of plasma F VIII:RAg studied by electroelution and immunoperoxidase detection. Thromb Res 43:579-84 (1986). <PUBMED:3092398>Cejka J Enzyme immunoassay for factor VIII-related antigen. Clin Chem 28:1356-8 (1982). <PUBMED:6804126>Sehested M & Hou-Jensen K Factor VII related antigen as an endothelial cell marker in benign and malignant diseases. Virchows Arch A Pathol Anat Histol 391:217-25 (1981). <PUBMED:6784337>

Additional Info

Recognizes the human Factor VIII Related Antigen. Specifically reacts with the cytoplasm of human endothelial cells from normal and neoplastic blood and from lymphatic vessels. Also reacts with human endocardium, platelets and megakaryocytes. Factor VIII R:Ag endocytosed by other cells may also react.