Factor X (HRP)

Factor X (F.X) is a vitamin K-dependent glycoprotein produced in the liver. The concentration of F.X in plasma is ~10ug/ml (~170nM). Factor X is expressed as a two-chain molecule with a molecular weight of 59kD. The light chain (17kD) of F.X contains a calcium-binding domain consisting of one hydroxyaspartic acid and 11 gamma carboxyglutamic acid (gla) residues. These residues allow F.X to bind to membranes that contain acidic phospholipids in a calcium dependent manner. This is followed by two EGF-like domains. The heavy chain of F.X (42kD) consists of the catalytic domain, carbohydrate and the activation peptide. Activation of F.X to the active enzyme (F.Xa) results from cleavage at residue Arg52 in the heavy chain of F.X by a complex of F.IXa, cofactor VIIIa, calcium and negatively charged phospholipid surface (the tenase complex), or by the F.VIIa-tissue factor complex. Both activation pathways result in the release of the activation peptide from the N-terminal of the heavy chain. The F.Xa generated is a serine protease responsible for the activation of prothrombin to thrombin in the presence of a phospholipid membrane, calcium and cofactor Va. The activity of F.Xa in plasma is inhibited by antithrombin (ATIII), alpha1-antitrypsin, alpha2-macroglobulin and tissue factor pathway inhibitor (TFPI). The inhibitory activity of ATIII is stimulated ~1000-fold by heparin.\n\nApplications:\nSuitable for use in Immunoelectrophoresis and ELISA. Other applications not tested.\n\nRecommended Dilutions:\nOptimal dilutions to be determined by the researcher.\n\nRz Ratio (Reinheitszahl A403/A280): 0.48\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

F0018-04

Size

200ug

Applications

ELISA

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in a buffered stabilizer solution, 50% glycerol. No preservatives added. Labeled with Horseradish Peroxidase (HRP).

P Type

Pab

Purity

Purified

Isotype

IgG

References

1. Ichinose, A., Davie, E.W., The Blood Coagulation Factors: Their cDNAs, Genes, and Expression; in Hemostasis and Thrombosis, 3rd Edition, pp 19-54, J.B. Lippincott Co., Philadelphia PA, USA (1994). 2. Steinberg, M., Nemerson, Y., The Activation of Factor X; in Hemostasis and Thrombosis, pp 91-99, J.B. Lippincott Co., Philadelphia PA, USA (1982). 3. Ellis, V., Scully, M., MacGregor, I., Kakkar, V., Inhibition of Human Factor Xa by Various Plasma Protease Inhibitors; Biochimica et Biophysica Acta 701: 24-31 (1982).

Additional Info

Recognizes human Factor X.