Factor XII (Hageman Factor)

Factor XII (F.XII, Hageman factor) is a 76kD, single chain glycoprotein produced in the liver. In plasma, F.XII circulates as a protease zymogen at a concentration of approximately 30ug/ml (400nM). Upon vascular injury F.XII binds to negatively charged extravascular surfaces such as cartilage and skin, which facilitate activation of the zymogen to the active serine protease. Cleavage of F.XII by kallikrein after residue Arg353 produces the enzyme alpha-F.XIIa, consisting of a 28kD light chain containing the protease domain, and a 52kD heavy chain containing the anionic surface-binding domain. Substrates for surface bound F.XIIa include the zymogens prekallikrein (PK) and factor XI (F.XI) as well as the procofactor high-molecular weight kininogen (HK). The activation of these substrates results in positive feedback activation of F.XII. Further cleavage of alpha-F.XIIa by kallikrein produces the 28kD fragment beta-F.XIIa (Hageman factor fragment). Beta-F.XIIa has reduced procoagulant activity as it lacks the anionic surface-binding domain, but is capable of fluid-phase activation of PK, factor VII and complement C1. The activity of F.XIIa in plasma is regulated predominantly by C1-Inhibitor, with relatively minor contributions by alpha2-antiplasmin, alpha2-macroglobulin and antithrombin, even in the presence of therapeutic levels of heparin (1-3).\n\nApplications:\nSuitable for use in ELISA, Immunoelectrophoresis and Immunodiffusion (vs. citrated plasma). Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F0019-06

Size

500ug

Applications

ELISA

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in 0.01M HEPES, pH 7.4, 0.15M sodium chloride, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. DeLa Cadena, R., et al., in Hemostasis and Thrombosis, 3rd ed. Colman, R.W., et al., eds. pp. 219-240. J.B. Lippincott Co., Philadelphia (1994). 2. Tankersley, D.L., et al., Thromb. Res. 25: 307-317 (1982). 3. Pixley, R.A., et al., J. Biol. Chem. 260: 1723-1729 (1985).

Additional Info

Recognizes human Factor XII. Species Crossreactivity: Reacts less intensely with samples from rabbit, mouse, rat and canine.