FAK (Focal Adhesion Associated Protein Tyrosine Kinase, BC3)

Epitope localizes in the C-terminal (aa 853-1052) of human FAK protein. FAK is a widely expressed non-receptor protein tyrosine kinase (PTK). FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Studies have shown that adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis. Excellent for multiple biochemical applications.\n\nApplications\nSuitable for use in Immunofluorescence, Immunoprecipitation and Western Blotting. Other applications have not been tested.\n\nRecommended Dilution:\nImmunoprecipitation: 10ug/mg protein lysate. Use Protein A.\nWestern Blotting: 5-10ug/ml for 2hrs at RT\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHUVEC or IMR-5 cells .\n\nCellular Localization:\nCytoplasmic and focal contacts\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F0019-54

Size

500ul

Applications

IF, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Can

Form

Supplied as a liquid in 10mM PBS, pH 7.4, 0.09% sodium azide, 0.2% BSA. Also supplied without BSA and sodium azide, cat#F0019-54X.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Hanks, S.K, Polte, T.R., Bioessays 19(2): 137-145, 1997.\n2. Ilic, D., et al., Journal of Cell Science 110: 401-407, 1997.\n3. Owens, L.V., et al., Cancer Research 55(13): 2752-2755, 1995.

Additional Info

Recognizes a 125kD protein, which is identified as FAK (Focal Adhesion Associated Protein-Tyrosine Kinase) of human, mouse, rat, canine and frog.