FAK, phosphorylated (Tyr925)

Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in integrin-mediated signal transduction. It plays an important role in the control of several biological processes, including cell spreading, migration and survival. Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397, which is a binding site for Src family kinases, PI3K and PLCgamma. The recruitment of Src family kinases results in the phosphorylation of tyrosine residues 407, 576 and 577 in the catalytic domain, and tyrosine residues 871 and 925 in the C-terminal region of FAK. Phosphorylation of Tyr925 creates a binding site for the Grb2/SH2 domain and triggers a Ras-dependent activation of the MAP kinase pathway.\n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunofluorescence. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000\nImmunoprecipitation: 1:100\nImmunofluorescence (IC): 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

F0019-58U

Size

100ul

Applications

IF, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by Protein A and peptide affinity chromatography.

Isotype

IgG

References

US Biological Application Reference: 1. Gonzalez, L., et al., J. Biol. Chem. 281: 20,851-20,864 (2006). General References: 1. Parsons, J.T., et al., Oncogene 19: 5606

Additional Info

Recognizes endogenous levels of human FAK, phosphorylated (Tyr925) at ~125kD. Species Sequence Homology: mouse, rat and chicken.