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FAK, phosphorylated (Tyr925)

FAK, phosphorylated (Tyr925)

Cat no: F0019-58U

Supplier: United States Biological
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Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in integrin-mediated signal transduction. It plays an important role in the control of several biological processes, including cell spreading, migration and survival. Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397, which is a binding site for Src family kinases, PI3K and PLCgamma. The recruitment of Src family kinases results in the phosphorylation of tyrosine residues 407, 576 and 577 in the catalytic domain, and tyrosine residues 871 and 925 in the C-terminal region of FAK. Phosphorylation of Tyr925 creates a binding site for the Grb2/SH2 domain and triggers a Ras-dependent activation of the MAP kinase pathway. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunofluorescence. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (IC): 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: F0019-58U
Reactivities: Human
Hosts: Rabbit
Applications: Immunofluorescence, Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by Protein A and peptide affinity chromatography.
References: US Biological Application Reference: 1. Gonzalez, L., et al., J. Biol. Chem. 281: 20,851-20,864 (2006). General References: 1. Parsons, J.T., et al., Oncogene 19: 5606
Additional info: Recognizes endogenous levels of human FAK, phosphorylated (Tyr925) at ~125kD. Species Sequence Homology: mouse, rat and chicken.

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