BRCA1-associated C-terminal helicase1 (BACH1) is a 82kD nuclear transcription regulatory protein belonging to the bZIP family. BACH1 contains basic leucine zipper and BTB-zinc finger domains which are directly involved in DNA binding and for Transcription regulation. BACH1 is found in a wide range of tissues and acts as a transcriptional regulator. It plays an important role in the coordination of transcription activation and repression by MAFK.
Applications:
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilutions:
Immunohistochemistry (Paraffin): 5ug/ml Requires antigen retrieval using heat treatment prior to staining paraffin sections. Sodium citrate buffer, pH 6.0 is recommended.
ELISA: 1:10,000-1:44,000
Western Blot: 1:500-1:2000
Optimal dilutions to be determined by the researcher.
Immunohistochemistry Positive Control:
Human skeletal muscle
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.