FAS (CD95) (TNF Receptor Superfamily, Member 6)

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform. \n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications have not been tested. \n\nRecommended Dilutions: \nPeptide ELISA Titer: 1:32,000\nWestern Blot: 0.1-0.3ug/ml detects a ~37kD band observed in lysates of cell line MOLT4 (calculated MW of 37.7kD according to NP_000034.1)\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

F0019-61R

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by peptide affinity chromatography.

Isotype

IgG

References

1.Blomberg J, Ruuth K, Jacobsson M, H

Additional Info

Recognizes human FAS. Expected to recognize reported isoforms 1 (NP_000034.1), 2 (NP_690610.1) and 3 (NP_690611.1).

Alternative Names

tumor necrosis factor receptor superfamily, member 6, tumor necrosis factor receptor superfamily member 6, apoptosis antigen 1, OTTHUMP00000059646, OTTHUMP00000020051, OTTHUMP00000020046, OTTHUMP00000020045, Fas antigen, Fas AMA, CD95 antigen, APO-1 cell surface antigen, TNFRSF6, FASTM, FAS1, CD95, APT1, APO-1, ALPS1A, Fas (TNF receptor superfamily, member 6), FAS