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Fas Ligand (FASL)

Cat no: F0019-65C

Fas Ligand (FASL)

Fas ligand is a cell surface molecule belonging to the tumor necrosis factor family, binds to its receptor Fas, thus inducing apoptosis. Various cells express Fas, where Fas ligand is expressed predominantly on activated T cells. Fas and Fas ligand are involved in the down-regulation of immune reactions as well as T cell-mediated cytotoxicity.\n\nComments: This antibody is excellent for staining of formalin-fixed, paraffin-embedded tissues.\n\nEpitope: N-terminus\n\nApplications:\nWestern Blot: (2-4ug/ml for 2 hours at RT)\nImmunohistology: (Formalin/paraffin) (Ab 1:50 to 1:100 for 30 min at RT) Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.\n\nPositive Control: Prostate\n\nCellular Localization: Cell membrane and cytoplasmic\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degrees C or colder. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F0019-65C

Size

250ul

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA and 15mM sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Additional Info

Recognizes Fas Ligand. Species Crossreactivity: Human, Mouse and Rat.

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
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