CD178 cell surface antigen is a 40kD glycoprotein also known as Fas ligand (CD95L). CD178 is a member of the TNF family, which is expressed by activated T lymphocytes and NK cells. The protein may exist as either a membrane bound or a cleaved soluble form. CD178 plays an important role in T cell development and cytotoxicity. Binding of CD178 to Fas (CD95) results in the induction of apoptosis.
Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1:500-1:3000
Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.