Fatty Acid Amide Hydrolase (FAAH)

Cannabinoids, a group of C21 compounds present in Cannabis sativa L., their carboxylic acids, analogs, and transformation products, are the active ingredients found in hasish and marihuana. (-)-trans-D9-tetrahydrocannabinol (D9-THC) is the major psychopharmacologically active component of Cannabis. Cannabis affects cognition and memory, euphoria and sedation, and antinociception (analgesia) without the respiratory depression problems associated with opioid analgesics. To date, two sub-types of the G-protein coupled cannabinoid receptor, CB1 and CB2, have been identified. The first brain-derived endogenous cannabinoids, an unsaturated fatty-acid ethanolamide, arachidonylethanolamide (AEA, also called anandamide) was found in brain. AEA has higher affinity for the CB1 than for the CB2. Neurons and astrocytes have been found to re-uptake and hydrolyse anandamide rapidly, resulting in the formation of arachidonic acid and ethanolamine. The uptake mechanism has been shown to be mediated by a saturable, selective, temperature-dependent and Na+-independent transporter. Anandamide hydrolysis is catalyzed by a membrane-bound amidohydrolase (called anandamide amidohydrolase or fatty acid amide hydrolase, FAAH). FAAH (rat/mouse/human 579aa; chromosome 1p34-p35; mol wt ~67 kD) sequence analyses suggest a single predicted transmembrane domain at the extreme N-terminus of the enzyme.\n\nApplications:\nSuitable for use in Western Blot and ELISA. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-10ug/ml (using Chemiluminescence technique).\nELISA: 1:10,000-1:50,000 using 50-100ng F0019-68A control peptide/well.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

F0019-68

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.1% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Patricelli, M.R. et al., Biochemistry, 37, 15177-15187 (1998). 2. Maccarone, M., et al., J. Biol. Chem., 273, 32332-32339 (1998). 3. Cravatt, B.F., et al., Nature, 384, 83-87 (1996). 4.

Additional Info

Recognizes human Fatty Acid Amide Hydrolase (FAAH). The immunogen shows no significant sequence homology with other hydrolases. Species Sequence Homology: rat-100%, mouse and porcine-94%, chicken-58%.