Fatty Acid Binding Protein 4, Recombinant, Human (FABP4, A-FABP)

Adipocyte fatty acid binding protein FABP4 is a 15kD member of the intracellular fatty acid binding protein (FABP) family, which is known for the ability to bind fatty acids and related compounds (bile acids or retinoids) in an internal cavity. FABP4 is expressed in a differentiation-dependent fashion in adipocytes and is a critical gene in the regulation of the biological function of these cells. In mice, targeted mutations in FABP4 provide significant protection from hyperinsulinemia and insulin resistance in the context of both dietary and genetic obesity. Adipocytes obtained from FABP4-deficient mice also have reduced efficiency of ipolysis in vitro and in vivo, and these mice exhibited moderately improved systemic dyslipidemia. Recent studies also demonstrated FABP4 expression in macrophages upon differentiation and activation. In these cells, FABP4 modulates inflammatory responses and cholesterol ester accumulation, and total or macrophage-specific FABP4 deficiency confers dramatic protection against atherosclerosis in the apoE-/- mice. These results indicate a central role for FABP4 in the development of major components of the metabolic syndrome through its distinct actions in adipocytes and macrophages.\n\nDescription: \nRecombinant Human Fatty Acid Binding Protein 4 is a monomeric ,non-glycosylated, polypeptide chain containing 132 amino acids and having a molecular mass of 14,700 Daltons.\n\nSpecificity: \nThe amino acid sequence of the recombinant human FABP4 is 100% homologous to the amino acid sequence of the human FABP4.\n\nApplications: \nWestern blotting, ELISA\n\nReconstitution:\n0.1M Acetate buffer pH4 and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 g/ml. In higher concentrations the solubility of this antigen is limited.\n\nStorage: \nStore lyophilized protein at -20 degrees C. Aliquot the product after reconstitution to avoid freeze-thaw cycles. Reconstituted protein can be stored at 4 degrees C for a limited period of time; it does not show any change after two weeks at 4 degrees C.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

F0019-82D

Size

2ug

Form

Supplied as a lyophilized powder in 0.05M acetate buffer, pH 4.0.

Purity

(same/more than) 90% (SDS-PAGE); Two-step procedure using size exclusion chromatography before and after refolding.

References

1. Identification of single nucleotide polymorphism of adipocyte fatty acid-binding protein gene and its association with fatness traits in the chicken.\n\nPoult Sci 2006 Mar;85(3):429-34\n\n2. Adipocyte fatty acid-binding protein is a plasma biomarker closely associated with obesity and metabolic syndrome.\n\nClin Che m 2006 Mar;52(3):405-13\n\n3. Adipocyte fatty acid-binding protein expression and lipid accumulation are increased during activation of murine macrophages by toll-like receptor agonists.\n\nArterioscler Thromb Vasc Biol 2005 Jun;25(6):1220-4\n\n4. Cloning and characterization of chicken adipocyte fatty acid binding protein gene.\n\nAnim Biotechnol 2004;15(2):121-32\n\n5. Adipocyte-fatty acid binding protein induces apoptosis in DU145 prostate cancer cells.\n\nJ Exp Ther Oncol 2004 Jul;4(2):91-100\n\n6. Physical association between the adipocyte fatty acid-binding protein and hormone-sensitive lipase: a fluorescence resonance energy transfer analysis.\n\nJ Biol Chem 2004 Dec 10;279(50):52399-405