Fatty Acid Binding Protein, Adipocyte (FABP)

Fatty acids are important for general cellular metabolism. A number of proteins have been implicated in the transport and storage of fatty acid. FABPs (fatty acid binding proteins) are a group of cytoplasmic, small mol wt (14-15kD) and proteins that has widespread tissue distribution. FABPs can bind long-chain fatty acid, fatty-acid acyl-CoA and acyl-L-carnitine. Several different isoforms of FABP have been identified and generally referred to by tissue type (liver, heart, intestine, adipocyte, kidney, brain etc; protein designated as H-FABP indicates that it is heart type). However, expression of these isoforms is not exclusive and more than one isoforms can be found in a given cell or tissue. FABPs share significant degree of sequence homology. FABPs are also known as mammary derived growth inhibitor (MDGI), adipocyte lipid binding protein (ALBP), Myelin protein P2 homolog, P2 adipocyte protein, 422 protein (P15).\n\nApplications: \nSuitable for use in Western Blot and ELISA. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:5000\nELISA: 1:10,000-1:50,000.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Control Peptide:\nF0019-77M: Fatty Acid Binding Protein, Human, Heart, Adipocyte, Human (FABP) Western Blot Control.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F0019-73H

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Bernlohr DA et al (1984) PNAS 81, 5468; 2. Phillips M et al (1986) JBC 261, 10821; 3. Hunt CB et al (1986) PNAS 83, 3786; 4. Cook JS et al (1988) PNAS 85, 2949;5. Xu Z et al (1993) JBC 268, 7874; 6. Baxa CA et al (1989) Biochem. 28, 8683; 7. Peter RA et al (1991) Biochem J 276, 203-20 8. Bas NM (1988) Int Rev Cytol 111, 143-184; 9. Bass Nm et al (1989) BBRC 137, 929-935; 10. Glatz JFC et al (1989) Mol Cell Biochem. 88, 37-44; 11. Matarese V et al (1988) JBC 263, 14544-14551

Additional Info

Recognizes rat adipocyte FABP. Species sequence homology: mouse AFABP/adipocyte P2: 90%, mouse myelin P2 protein homolog: 85%, human AFABP: 80%, bovine AFABP: 65%, human P2 protein homolog: 55%, human Epidermal FABP/Psoriasis-associated binding protein homolog (PA-FABP): 55%, rat testis lipid binding protein: 57% and mouse PA-FABP: 45%.