Fatty Acid Binding Protein, Intestinal (FABP)

Intestinal fatty acid binding protein (I-FABP) is specific localized in the small bowel epithelium. I-FABP is detectable in serum and urine after ischemic injury to the small bowel.\n\nApplications:\nSuitable for use in staining cells containing I-FABP in cells cultured on cover slip (Immunohistochemistry), Western Blot and ELISA. \n\nRecommended Dilution:\nImmunohistochemistry: 1:10\nWestern Blot: 1:10\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F0019-76G

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Prc, Sheep

Form

Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Lieberman, J., et al., Surgery 121: 335 (1997). 2. Morrissey, P., et al., Transplantation 61: 1451 (1996). 3. Kanda, T., et al., Gastroenterology 110: 339 (1996). 4. Kanda, T., et al., Gut 36: 788 (1995).

Additional Info

Recognizes human intestinal fatty acid binding protein (I-FABP) of both natural and recombinant origin. Specificity was further assessed by ELISA and Western Blot. No crossreactivities with other FABP types have been detected. Species Crossreactivity: rat, mouse, sheep and porcine