Fatty Acid, Free BioAssay(TM) Kit

Fatty Acids play very important roles in normal metabolism and many disease development.\nThey are precursors to a number of bioactive classes of compounds such as prostaglandins,\nleucotrienes and others, and have been implicated in diverse functions such as autism, immune system and inflammation response. The Free Fatty Acid Quantification Kit provides a convenient, sensitive enzyme-based method for detecting the long-chain free fatty acids in various biological samples, such as serum, plasma and other body fluids, food, growth media, etc. In the assay, Fatty Acids are converted to their CoA derivatives, which are subsequently oxidized with the concomitant generation of color or fluorescence. C-8 (octanoate) and longer fatty acids can then be easily quantified by either colorimetric (spectrophotometry at lambda = 570nm) or fluorometric (at Ex/Em = 535/587nm) methods with detection limit 2uM free fatty acid in variety samples.\n\nKit Components:\n*F0019-94A: Assay Buffer, 1x25ml\nF0019-94B: Probe, 1x200ul\nF0019-94C: ACS Reagent, 1x1vial \nF0019-94D: Enzyme Mix, 1x1vial\nF0019-94E: Enhancer, 1x200ul\nF0019-94F: Palmitic Acid Standard (1nmol/ul), 1x300ul\n\nStorage and Stability:\nStore the kit at -20 degrees C. Protect from light. Allow *F0019-984A: Assay Buffer to warm to RT before use. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts