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FceRI, alpha (Fc epsilon RI alpha, FCE1A, FCER1A, High Affinity Immunoglobulin epsilon Receptor Subunit alpha, IgE Receptor)

FceRI, alpha (Fc epsilon RI alpha, FCE1A, FCER1A, High Affinity Immunoglobulin epsilon Receptor Subunit alpha, IgE Receptor)

Cat no: F0025-06B1

Supplier: United States Biological
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Applications: Suitable for use in ELISA, Flow Cytometry, Immunofluorescence, Immunoprecipitation, and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:25-1:50; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: F0025-06B1
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot
Size: 200ug
Form: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
P type: Mab
Isotype: IgG2b
Purity: Purified by Protein G affinity chromatography.
Alternative names: Fc Fragment of IgE, High Affinity I, Receptor for alpha polypeptide; IgE Fc Receptor Subunit alpha; Fc-epsilon RI-alpha
References: 1. Vangelista, L. et al. (2002) A minimal receptor-Ig chimera of human Fc epsilon R1 alpha-chain efficiently binds secretory and membrane IgE. Protein Engineering. 15: 51-57, 2. Vangelista, L. et al. (2002) Efficient folding of the Fc epsilonR1 alpha-chain membrane-proximal domain D2 depends on the presence of the N-terminal domain D1. J. Mol. Biol. 322: 815-25.
Additional info: Recognizes human Fc Epsilon R1, alpha.

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