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FceRI, alpha (Fc epsilon RI, IgE Receptor)

Cat no: F0025

FceRI, alpha (Fc epsilon RI, IgE Receptor)

The allergic reaction is mediated through the IgE high affinity receptor (FceRI). When a multivalent antigen is presented, a redistribution of the monomeric IgE-FceRI receptor complexes cause the degranulation of mast cells and basophils, resulting in the subsequent release of factors responsible for the allergic reaction. The FceRI receptor is a tetrameric complex, consisting of an a-chain, a b-chain and a dimeric g-chain. While the a-chain is primarily involved with the binding of IgE, the signal is transferred through the g subunit, which contains the immunoreceptor tyrosine activation motifs (ITAM) critical for initiating receptor mediated signal transduction through the Syk and Lyn tyrosine kinases. The FceRI receptor is able to engage and disengage the IgE, providing a versatile model for studying the function of kinase coupled receptors.\n\nApplications:\nSuitable for use in Immunoprecipitation/Western Blot and FACS. Not suitable for Western Blot without pre-Immunoprecipitation of antibody. Other applications have not been tested.\n\nRecommended Dilutions: \nImmunoprecipitation: 5ug immunoprecipitates rat FceRI alpha from 500ug of biotinylated rat basophilic leukemia cell lysate. The immunoprecipitate was resolved by electrophoresis and transferred to nitrocellulose. Biotinylated protein bands were visualized using NeutrAvidin labeled with HRP and an enhanced chemiluminescence detection system. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

F0025

Size

200ug

Applications

FC, IP

Hosts

Mouse

Reactivities

Rat

Form

Supplied as a liquid in 0.1M Tris-glycine, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

1. Letourneur, O., et al., J. Biol. Chem. 270: 8249-8256 (1995).

Additional Info

Recognizes rat FceRI alpha at ~45-55kD.

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