FCRL4, also known as IRTA1 (immunoglobulin superfamily receptor translocation associated 1), is a member of the Ig superfamily. It shares sequence homology with the classical Fc receptors. FCRL4 is preferentially expressed in B cells and contains three potential ITIM motifs in its extracellular domain. The gene for FCRL4 is localized to the human chromosome Iq 21-23 region, a hotspot for translocation events.
Applications:
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilutions:
Flow Cytometry: 10ul labels 1-2.5x10e5 RPMI-8226 cells.
Optimal dilutions to be determined by the researcher.
Flow Cytometry Protocol:
1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction.
2. After blocking, 10ul of F0025-75 was added to 1-2.5 x 10e5 cells and incubated for 30 minutes at RT.
3. Unbound antibody was removed by washing the cells twice in Flow Cytometry Staining Buffer (Catalog # F4568-10). Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer.
4. The cells were resuspended in F4568-10 for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with APC-labeled mouse IgG1 antibody.
Storage and Stability:
May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing Allophycocyanin conjugates will result in a substantial loss of enzymatic activity.
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