FCRL5 (Fc Receptor-like Protein 5, FcR-like Protein 5, BXMAS1-like Protein 2, mBXMH2, Fc Receptor Homolog 3, FcRH3, moFcRH3, CD307e) (APC)

Fc Receptor-Like 5 (FCRL5), also known as FcRH3 (FcRH5 in human), IRTA2, and CD307e, is a 90-95kD member of the FCRL family of proteins whose aa sequence is reminiscent of classical Fc receptors. FCLR molecules are type I transmembrane proteins that contain from three to nine Immunoglobulin-like domains. They are differentially expressed within the B cell lineage, and can either promote or inhibit B cell proliferation and activation. Mature mouse FCRL5 consists of a 470aa extracellular domain (ECD), a 21aa transmembrane segment, and a 79aa cytoplasmic region. FCRL5 expression is restricted to mature B lineage cells in lymphoid tissues and blood, and is particularly noted to be expressed on T-independent marginal zone and B1 B cells. Its ligation inhibits signaling through the B cell antigen receptor. Epstein-Barr virus transformation of B cells induces the up-regulation of surface FCRL5 by a direct effect of its EBNA2 protein on FCRL5 gene transcription. FCRL5 on B cells functions as a receptor for the orthopoxvirus MHC class I-like protein OMCP. Based on the literature and testing, both mouse and human FCRL5 will bind to purified IgG with high affinity.\n\nApplications:\nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nAbsorption: 620-650nm\n\nEmission: 660-670nm\n\nStorage and Stability:\nMay be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. APC is light sensitive.

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SPECIFICATIONS

Catalog Number

F0025-76C

Size

100Tests

Applications

FC

Hosts

Sheep

Reactivities

Mouse

Form

Supplied as a liquid in saline, 0.5% BSA, 0.1% sodium azide. Labeled with Allophycocyanin (APC).

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Davis, R.S. (2007) Annu. Rev. Immunol. 25:525. 2. Maltais, L.J. et al. (2006) Nat. Immunol. 7:431. 3. Won, W-J. et al. (2006) J. Immunol. 177:6815. 4. Davis, R.S. et al. (2004) Int. Immunol. 16:1343. 5. Hatzivassiliou, G. et al. (2001) Immunity 14:277. 6. Miller, I. et al. (2002) Blood 99:2662. 7. Polson, A.G. et al. (2006) Int. Immunol. 18:1363. 8. Vidal-Laliena, M. et al. (2005) Cell. Immunol. 236:6. 9. Haga, C.L. et al. (2007) Proc. Natl. Acad. Sci. USA 104:9770. 10. Mohan, J. et al. (2006) Blood 107:4433. 11. Campbell, J.A. et al. (2010) J. Immunol. 185:28.

Additional Info

Recognizes mouse FCRL5.