Ferric Reducing Ascorbate (FRASC) BioAssay(TM) Kit

Ascorbic Acid (Vitamin C) plays an important role in many biological processes. It is a potent anti-oxidant, anti-inflammatory, anti-viral agent and an immune stimulant and is present in a wide variety of biological specimens. Due to the presence of a variety of other antioxidants in biological samples such as serum, most ascorbic acid assays show strong interference. The FRASC Assay Kit provides a rapid, simple, and sensitive means of detecting ascorbic acid in biological samples such as serum and other body fluids, tissue and cell extracts, growth media and food products. In this assay, Fe+3 is reduced to Fe+2 by any antioxidants present. The ferrous iron is chelated with a colorimetric probe to produce a product with a strong absorbance band which can be monitored between 545-600nm. The addition of ascorbate oxidase to parallel samples removes any ascorbate present leaving a background value which is subtracted from the total to give ascorbate content. The assay can detect 0.2 to 20nmol of ascorbic acid in various samples.\n\nKit Components:\n*FRASC Buffer 1x25ml\nAscorbic Acid Probe 1x1ml\nFeCl3 solution 1x1ml\nAscorbate Oxidase (lyophilized) 1x1vial\nAscorbic Acid Standard (20umole) 1x1vial.\n\nStorage and Stability:\nStore the kit at -20 degrees C. Allow *FRASC Buffer to warm to RT before use. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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