Ferritin H-chain (FTH)

Elemental iron is required for a variety of normal cellular functions. It is vital for proper growth and development. Ferritin is the major protein involved in iron sequestration and detoxification. Ferritin is found in all living species. Mammalian liver and spleen ferritin (~450kD) consists of 24 subunits of 2 species, the heavy subunit (~21kD; FTH) and the light subunit (~19kD; FTL). The 2 types of apoferritin subunits were designated H and L for heart and liver, respectively. Ferritin molecules from plants and bacteria contain only H-type chains. 'H-type' is associated with the presence of centers catalyzing the oxidation of two Fe(II) atoms. FTL subunit is coded by a gene in segment 19q13.3. FTH subunit is located on chromosome 11. Ferritin is capable of storing up to 4500 atoms of ferric iron. The H-to-L ratio within ferritin varies in a tissue-specific manner. This ratio is also influenced by pathophysiological conditions, including inflammation and malignancy. Hyperferritinemia-cataract syndrome has a mutation in the iron response element (IRE) in the 5-prime noncoding region of the FTL gene. Synthesis of both ferritin subunits is controlled by a common cytosolic protein, iron regulatory proteins (IRPs), which binds to the iron-responsive element (IRE) in the 5'-UTR of the H- and L-ferritin mRNAs. H-chains are important for Fe(II) oxidation. L-chains assist in core formation.\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:5000 using ECL.\nELISA: 10,000-50,000. Control peptide can be used to coat plates at 1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide:\nF4015-50: Ferritin H-chain, Human, Control Peptide (FTH)\nF4015-55: Ferritin H-chain, Human, Western Blot Positive Control (FTH)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

F4015-45

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Boyd, D., et al., JBC 260: 11755 (1985). 2. Costanzo, F., et al., EMBO J. 3: 23 (1984). 3. Chou, C.C., et al., Mol. Cell. Biol. 6: 566-573 (1986). 4. Hentze, M.W., et al., PNAS 83: 722 (1986). 5. Harrsion, P.M., et al., BBA 1275: 161 (1996). 6. Picard, V., et al., JBC 273: 15382 (1998). 7. Rucker, P-F., et al., JBC 271: 33352 (1996). 8. Nelson, N., et al., EMBO J. 18: 4361(review) (1999). 9. Cairo, G., et al., Biochem. J. 352: 241-250 (2000).

Additional Info

Recognizes Human FTH heavy chain. No sequence homology with ferritin L-chain. Sequnce Homology: Human FTL, 53%. Species Sequence Homology (100%): mouse, rat, bovine, monkey, chicken