Fetuin, Human (alpha-2 Heremans-Schmid Glycoprotein, FHSG, Fetuin A)

Fetuin is a liver-produced negative acute phase protein composed of two subunits, the A and B chains. Fetuin homologs have been identified in several species including rat, sheep, pig, rabbit, guinea pig, cattle, mouse and human. Multiple physiological roles for these homologs have been suggested, including ability to bind to hydroxyapatite crystals and to specifically inhibit the tyrosine kinase (TK) activity of the insulin receptor (IR). Fetuin-A (alpha2-Heremans-Schmid glycoprotein; AHSG) is an important circulating inhibitor of calcification in vivo, and is downregulated during the acute-phase response. Sera from patients on long-term dialysis with low AHSG concentrations showed impaired ex-vivo capacity to inhibit CaxPO4 precipitation. Fetuin may influence the resolution of inflammation by modulating the phagocytosis of apoptotic cells by macrophages. ASHG blocks TGF-beta-dependent signaling in osteoblastic cells, and mice lacking ASHG display growth plate defects, increased bone formation with age, and enhanced cytokine-dependent osteogenesis.\n\nApplications:\nSuitable for use in Western Blot and ELISA. Other applications not tested.\n\nStorage and Stability: \nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with ddH2O. Aliquot and store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\n\nReconstitution: \nAdd 0.2 ml of deionized H2O and let the lyophilized pellet dissolve completely.\n\nStorage: \nStore lyophilized protein at -20 degrees C. Aliquot the product after reconstitution to avoid freeze-thaw cycles. Reconstituted protein can be stored at 4 degrees C for a limited period of time; it does not show any change after two weeks at 4 degrees C.

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SPECIFICATIONS

Catalog Number

F4102-20

Size

10ug

Form

Supplied as a lyophilized powder in 0.02M Tris-HCl, pH 8.0, 0.2M sodium chloride. Reconstitute with 200ul of deionized H2O and let the lyophilized pellet dissolve completely.

Purity

(same/more than) 95% (SDS-PAGE)

References

1. Serum albumin, C-reactive protein, interleukin 6, and fetuin a as predictors of malnutrition, cardiovascular disease, and mortality in patients with ESRD. Am J Kidney Dis 2006 Jan;47(1):139-48 2. Imparting mineral affinity to fetuin by bisphosphonate conjugation: a comparison of three bisphosphonate conjugation schemes. Mol Pharm 2005 Sep-Oct;2(5):392-406 3. Cleavage of disulfide-linked fetuin-bisphosphonate conjugates with three physiological thiols. Biomacromolecules 2005 Sep-Oct;6(5):2800-8 4. Adsorption of serum fetuin to hydroxylapatite does not contribute to osteoblast phenotype modifications. J Biomed Mater Res A 2005 Apr 1;73(1):39-47 5. The serum glycoprotein fetuin-A promotes Lewis lung carcinoma tumorigenesis via adhesive-dependent and adhesive-independent mechanisms. Cancer Res 2005 Jan 15;65(2):499-506 6. Identification of Fetuin-B as a member of a cystatin-like gene family on mouse chromosome 16 with tumor suppressor activity. Genome 2004 Oct;47(5):931-46