FEZ1 (Fasciculation and Elongation Protein zeta 1, Zygin-1, Zygin I)

Mammalian FEZ1 is a membrane protein located primarily in the brain. It serves as a protein kinase C (PKC-Z) substrate. Following phosphorylation by PKC-Z, FEZ1 translocates from the plasma membrane to the cytoplasm where it interacts with proteins involved in axonal outgrowth and cellular transport. \n\nIn addition, FEZ1, interacts with proteins involved in transcriptional regulation and chromatin remodelling indicating that FEZ1 may posses nuclear regulatory functions along with those of neuronal development. \n\nStudies suggest that at least two distinct isoforms of FEZ1 exist. It should be noted that in spite of it predicted molecular weight, FEZ1 often runs at a higher molecular weight in SDS-PAGE.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F4112-50

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Blasius, T.L., et al. (2007) Two binding partners cooperate to activate the molecular motor kinesin-1. J. Cell Biol.; 176:11-7 2. Kuroda, S., et al. (1999) Mammalian homologue of the Caenorhabditis elegans UNC-76 protein involved in axonal outgrowth is a protein kinase C zeta-interacting protein. J. Cell Biol.; 144:403-11 3. Bloom, L., et al. (1997) The Caenorhabditis elegans gene unc-76 and its human homologs define a new gene family involved in axonal outgrowth and fasciculation. Proc. Natl. Acad. Sci. USA 1997; 88:9578-82.

Additional Info

Recognizes human FEZ1. Species Crossreactivity: mouse, rat.

Alternative Names

Fez