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FGF-18, Recombinant, Human (Fibroblast Growth Factor-18, zFGF5, FGFI)

Cat no: 143321

FGF-18, Recombinant, Human (Fibroblast Growth Factor-18, zFGF5, FGFI)

FGF-18 is a heparin binding growth factor that belongs to the FGF family.Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation.FGF-18 is an essential regulator of long bone and calvarial development.FGF-18 signals through FGFR 1c, 2c, 3c, and 4.Recombinant E.coli derived human FGF-18 is a 20.1kD protein consisting of 173 amino acid residues, resulting from c-terminal truncation of the full length protein.\n\nBiological Activity:\nThe ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors is ~0.5ng/ml, corresponding to a specific activity of ~2x10e6 units/mg.\n\nAA Sequence:\nAEENVDFRI HVENQTRARD DVSRKQLRLY QLYSRTSGKH IQVLGRRISA RGEDGDKYAQ LLVETDTFGS QVRIKGKETE FYLCMNRKGK LVGKPDGTSK ECVFIEKVLE NNYTALMSAK YSGWYVGFTK KGRPRKGPKT RENQQDVHFM KRYPKGQPEL QKPFKYTTVT KRSR\n\nCrossreactivity:\nMouse, Rat\n\nQuality Control:\nVerified by N-terminal and Mass Spectrometry analyses (when applicable).\n\nEndotoxin:\n<0.1ng/ug of protein (<1EU/ug).\n\nProtein Content:\nVerified by UV Spectroscopy and/or SDS-PAGE gel.\n\nStorage and Stability:\nStore lyophilized products at -20 degrees C. For reconstituted solutions of most products, we recommend short-term storage at 4 degrees C. For longer term storage the protein solution should be stored with a carrier protein (eg. 0.1% BSA) in working aliquots and stored frozen at -20 degrees C. Additional freeze/thaw cycles may cause some denaturation of the protein.

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SPECIFICATIONS

Catalog Number

143321

Size

5ug

Form

Supplied as a lyophilized powder.

Purity

(same/more than)95% by SDS-PAGE gel and HPLC analyses.

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