Home  >  Products  >  FHL1 (Four and a Half LIM Domains Protein 1, FHL-1, FHL1A, FHL1B, FLH1A, KYOT, MGC111107, Skeletal Muscle LIM-protein 1, SLIM1, SLIM-1, SLIM, SLIMMER, XMPMA)
FHL1 (Four and a Half LIM Domains Protein 1, FHL-1, FHL1A, FHL1B, FLH1A, KYOT, MGC111107, Skeletal Muscle LIM-protein 1, SLIM1, SLIM-1, SLIM, SLIMMER, XMPMA)

FHL1 (Four and a Half LIM Domains Protein 1, FHL-1, FHL1A, FHL1B, FLH1A, KYOT, MGC111107, Skeletal Muscle LIM-protein 1, SLIM1, SLIM-1, SLIM, SLIMMER, XMPMA)

Cat no: F4149-77G

Supplier: United States Biological
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HL1, a LIM domain containing protein abundantly expressed by skeletal muscle, which has been linked with several muscular dystrophy-like muscle disorders, including Scapuloperoneal syndrome (SPS), X-linked severe early-onset reducing body myopathy (RBM), and X-linked myopathy with postural muscle atrophy (XMPMA). Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: ELISA: 1:32,000 Western Blot: 0.05-3ug/ml detects a band of ~30kD in human muscle cell lysates. Immunohistochemistry (paraffin): 2-2.5ug/ml requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended Optimal dilutions to be determined by the researcher. Positive Control (Ihc): Human testis Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: F4149-77G
Reactivities: Human
Hosts: Goat
Applications: ELISA, Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris, pH 7.3, 0.05% BSA, 0.02% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by affinity chromatography.
Alternative names: Four and a Half LIM Domains Protein 1, FHL1 Protein, RP23-71G11.1
Additional info: Recognizes human FHL1. Expected to recognize FHL1 isoforms 5 and 2, the latter being implicated as a negative regulator of RBP J- mediated transcription. Species sequence homology: canine, mouse, rat, bovine, porcine.

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