Fibrillin 1

Fibrillin-1 is an extracellular acidic protein with a high cysteine content and an extended thread-like shape with mosaic composition of different types of extracellular modules. Most of the fibrillin molecule is contributed by 47 epidermal growth factor-like (EGF-like) repeats; 43 of them have a consensus sequence for calcium binding (cb). Other modules including 8-cysteine motifs (seven), hybrid motifs (two), and unique domains (three) are interspersed throughout the molecule. Fibrillin-1 contains a proline-rich region close to the N-terminus that is replaced by a glycine-rich region in fibrillin-2. The fibrillins are found throughout the connective tissue as integral components of extended fibrils. There is an aberrant expression of fibrillin-1 in Marfan syndrome.\n\nApplications: \nSuitable for use in Western Blot, Immunofluorescence, Immunoprecipitation and ELISA. Other aapplications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

F4195-02

Size

100ug

Applications

ELISA, IF, IP, WB

Hosts

Mouse

Reactivities

Hum, Bov, Ch/Bird

Form

Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.

P Type

Mab

Purity

Purified

Isotype

IgG1

References

1. Reinhardt, D., et al., J. Mol. Biol. 258: 104

Additional Info

Recognizes human Fibrillin-1. Epitope mapping studies identify the binding site of this antibody to the C-terminal end of the molecule, between aa 2093-2871. Species Crossreactivity: bovine and chicken.