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Fibrinogen (Coagulation Factor I) (FITC)

Fibrinogen (Coagulation Factor I) (FITC)

Cat no: F4199-91L

Supplier: United States Biological
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Fibrinogen (clotting factor I) is a heat labile beta glycoprotein present in plasma. It is the precursor of fibrin, which is the key protein constituting the network of the blood clot. Thrombin converts fibrinogen to fibrin by limited proteolysis. Fibrin monomers polymerize to fibrin which is stabilized by cross-linking. Applications: Suitable for use in ELISA, Immunoelectrophoresis and Radial Immunodiffusion. Other applications not tested. Recommended Dilution: ELISA: 1:20-1:80 Optimal dilutions to be determined by the researcher. Excitation: 492nm Emission: 515nm Fluorescein/IgG protein Molar Ratio (F/P): ~1.5 Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
Catalogue number: F4199-91L
Reactivities: Human, Bovine, Canine, Feline, Non-Human Primate
Hosts: Goat
Applications: ELISA
Size: 1ml
Form: Supplied as a lyophilized powder from PBS, pH 7.2. Preservative free. Labeled with fluorescein isothiocyanate (FITC). Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
P type: Pab
Isotype: IgG
Purity: Purified by DEAE chromatography.
Additional info: Recognizes human fibrinogen. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony) against normal human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified fibrinogen. No reaction is obtained with any other plasma protein component or serum. May also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products. Species Crossreactivity: Bovine, canine, feline, monkey

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