Fibrinogen, Human BioAssay(TM) ELISA Kit (Coagulation Factor I)

Fibrinogen is a soluble glycoprotein found in the plasma, with a molecular weight of 340kD. It comprises of three pairs of non-identical polypeptide chains (alpha, 63.5kD beta, 56kD, and gamma, 47kD chains) linked to each other by disulphide bonds. Low plasma fibrinogen concentrations are therefore associated with an increased risk of bleeding due to impaired primary and secondary hemostasis. Therefore Fibrinogen is an essential component of the blood coagulation system. Also it may play key roles in the process of atherosclerotic lesion formation, with subsequent effects on cardiovascular diseases. And increasing evidence from epidemiological studies suggests that elevated plasma fibrinogen levels are associated with an increased risk of ischaemic heart disease(IHD), stroke and other thromboembolism.\n\nIntended Use:\nHuman Fibrinogen ELISA kit is to be used for the in vitro quantitative determination of human fibrinogen in human serum, human plasma, cell lysate and buffered solution. The assay will recognize native human fibrinogen. This kit has been configured for research use only and is not to be used in diagnostic procedures.\n\nSensitivity:\nThe minimal detectable dose of human fibrinogen was calculated to be 7.63ng/ml, by subtracting two standard deviations from the mean of 12 zero standard replicates (ELISA buffer, S0) and intersecting this value with the standard curve obtained in the same calculation.\n\nSpecificity:\nThe following substances were tested and found to have no cross-reactivity: human serum albumin, transferrin, IgG, alpha-fetoprotein (AFP), plasminogen, Vitamin D binding protein (VDBP), Hemoglobin.\n\nTest Principle:\nThe design of this assay is based on a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to human Fibrinogen. Samples are pippetted into these wells. Nonbound fibrinogen and other components of the sample should be removed by washing, then biotin- conjugated monoclonal antibody specific to fibrinogen added. In order to quantitatively determine the amount of fibrinogen present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) should be added to each microplate well. The final step, a TMB-substrate solution added to each well. Finally, a sulfuric acid solution is added and the resulting yellow colored product is measured at 450nm. Since the increases in absorbency is directly proportional to the amount of captured fibrinogen.\n\nKit Components:\nMicroplate: 1x96 wells\nIncubation buffer: 1x30ml\nWashing buffer, (10x): 1x100ml\nStandard protein: 1 glass vial lyophilized\nStandard/sample dilution buffer: 1x25ml\nSecond antibody: 1 glass vial lyophilized\nAV-HRP: 1x150ul\nSecondary antibody/AV-HRP dilution buffer: 1x25ml\nSubstrate (TMB): 1x20ml\nStop solution: 1x20ml\n\nStorage and Stability:\nStore components at 4 degrees C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts