Fibroblast Growth Factor 19, Recombinant, Human (FGF19)

Human FGF-19 Fibroblast growth factor 19 (FGF-19) belongs to the large FGF family which has at least 23 members.1, 2 All FGF family members are heparin-binding growth factors with a core 120 amino acid (aa) FGF domain that allows for a common tertiary structure. FGFs are expressed during embryonic development and in restricted adult tissues. They act on cells of mesodermal and neuroectodermal origin to regulate diverse physiologic functions including angiogenesis, cell growth, pattern formation, embryonic development, metabolic regulation, cell migration, neurotrophic effects and tissue repair.3, 4 Signaling receptors for FGFs are type I\ntransmembrane receptor tyrosine kinases belonging to the Ig superfamily. Four distinct but related classes of FGF receptors, FGF R1, 2, 3, and 4, exist. Through alternative splicing, multiple isoforms for FGF R1, 2 and 3, with distinct ligand recognition profiles, are also generated.4\nHuman FGF-19 cDNA predicts a 251 aa precursor protein with a 22 aa signal peptide and a 229 aa secreted mature protein with no potential N-linked glycosylation sites.1, 2 Among FGF family members, human FGF-19 is most closely related chicken FGF-19 and murine FGF-15, sharing approximately 61% and 51% aa sequence identity, respectively.1, 2, 5 Neither the human orthologue of mouse FGF-15, nor the mouse counterpart of human FGF-19 has been identified. With the exception of adult gall bladder epithelium, FGF-19 expression is restricted to fetal tissues.1, 2 Unlike most FGFs which bind to and activate more than one FGF receptor, FGF-19 is a specific ligand for FGF R4.2 Similarly, another FGF family member, FGF-7 (KGF), only activates KGF R, the IIIb isoform of FGF R2.4 During chick embryogenesis, FGF-19 has been shown to act synergistically with Wnt-8c to initiate inner ear development.5\n\nSource: A DNA sequence encoding the mature human FGF-19 (Phe 27-Lys 216, Accession O95750) (Xie, M. et al., 1999, Cytokine 11:729-735)\n\nMolecular Mass: Based on N-terminal sequencing, the recombinant mature human FGF-19 has Phe 27 at the amino terminus and has a calculated molecular mass of 21kD.\n\nBiological Activity:\nFGF-19 is a high affinity heparin dependent ligand for FGFR4. Embryonic regions known to express FGF-19 include skin, cartilage, retina, gall bladder and small intestine. Activity measured by its ability to bind rhFGF R4/Fc in a functional ELISA. Immobilized rhFGF R4/Fc at 5ug/ml (100ul/well) can bind rhFGF-19 with a linear range of 3-200ng/ml.\n\nStorage and Stability:\nLyophilized samples are stable for up to six months at -20 degrees C to -70 degrees C. Upon reconstitution, this cytokine can be stored under sterile conditions at 2-8 degrees C for one month or at -20 degrees C to -70 degrees C in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

F4212-91B

Size

25ug

Form

Supplied as a lyophilized powder from a 0.2um filtered solution in PBS containing 50ug of bovine serum albumin per 1ug of cytokine. Reconstitute with sterile, ddH2O, 0.1-1% BSA to 0.1-1mg/ml.

Purity

(same/more than) 97% by SDS PAGE and HPLC analyses. Purified by sequential chromatography. Endotoxin: (same/less than) 0.1ng/ug

References

1. Nishimura, T. et al., 1999, Biocheim. Biophys. Acta 1444:148.\n2. Xie, M. et al., 1999, Cytokine 11:729.\n3. Goldfarb, M., 1996, Cytokine & Growth Factor Reviews 7:311.\n4. Green, P. et al., 1996, BioEssays 18:639.\n5. Ladher, R.K. et al., 2000, Science 290:1965.