Fibroblast Growth Factor 19, Recombinant, Human (FGF19)

Fibroblast Growth Factor-19, human, recombinant produced in E. coli is a single, non-glycosylated, polypeptide chain containing 206 amino acids and having a molecular mass of 23kD. The amino acid sequence of the recombinant human FGF19 is 100% homologous to the amino acid sequence of the human FGF19 without signal sequence and contains his tag at N-terminal The FGF-19 is purified by proprietary chromatographic techniques.\n\nHuman FGF-19 Fibroblast growth factor 19 (FGF-19) belongs to the large FGF family which has at least 23 members.1, 2 All FGF family members are heparin-binding growth factors with a core 120 amino acid (aa) FGF domain that allows for a common tertiary structure. FGFs are expressed during embryonic development and in restricted adult tissues. They act on cells of mesodermal and neuroectodermal origin to regulate diverse physiologic functions including angiogenesis, cell growth, pattern formation, embryonic development, metabolic regulation, cell migration, neurotrophic effects and tissue repair.3, 4 Signaling receptors for FGFs are type I\ntransmembrane receptor tyrosine kinases belonging to the Ig superfamily. Four distinct but related classes of FGF receptors, FGF R1, 2, 3, and 4, exist. Through alternative splicing, multiple isoforms for FGF R1, 2 and 3, with distinct ligand recognition profiles, are also generated.4\nHuman FGF-19 cDNA predicts a 251 aa precursor protein with a 22 aa signal peptide and a 229 aa secreted mature protein with no potential N-linked glycosylation sites.1, 2 Among FGF family members, human FGF-19 is most closely related chicken FGF-19 and murine FGF-15, sharing approximately 61% and 51% aa sequence identity, respectively.1, 2, 5 Neither the human orthologue of mouse FGF-15, nor the mouse counterpart of human FGF-19 has been identified. With the exception of adult gall bladder epithelium, FGF-19 expression is restricted to fetal tissues.1, 2 Unlike most FGFs which bind to and activate more than one FGF receptor, FGF-19 is a specific ligand for FGF R4.2 Similarly, another FGF family member, FGF-7 (KGF), only activates KGF R, the IIIb isoform of FGF R2.4 During chick embryogenesis, FGF-19 has been shown to act synergistically with Wnt-8c to initiate inner ear development.5\n\nAmino acid sequence:\nMRGSHHHHHH GMASLAFSDA GPHVHYGWGD PIRLRHLYTS GPHGLSSCFL RIRADGVVDC ARGQSAHSLLEIKAVALRTV AIKGVHSVRY LCMGADGKMQ GLLQYSEEDC AFEEEIRPDG YNVYRSEKHR LPVSLSSAKQ RQLYKNRGFL PLSHFLPMLP MVPEEPEDLR GHLESDMFSS PLETDSMDPF GLVTGLEAVR SPSFEK.\n\nReconstitution:\nReconstitute with sterile, ddH2O, 0.1-1% BSA to 0.1-1mg/ml. Add deionized water to a working concentration approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filte\n\nStorage and Stability:\nStable at room temperature for 3 weeks, should be stored desiccated below -20 degrees C. Upon reconstitution Fibroblast Growth Factor-19 should be stored at 4 degrees C between 2-7 days and for future use below -20 degrees C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

F4212-91C

Size

5ug

Form

Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 20mM Tris, 20mM sodium chloride, pH 7.5.

Purity

(same/more than) 95% by SDS PAGE and HPLC analyses. Purified by sequential chromatography. Endotoxin: (same/less than) 0.1ng/ug

References

1. Nishimura, T. et al., 1999, Biocheim. Biophys. Acta 1444:148.\n2. Xie, M. et al., 1999, Cytokine 11:729.\n3. Goldfarb, M., 1996, Cytokine & Growth Factor Reviews 7:311.\n4. Green, P. et al., 1996, BioEssays 18:639.\n5. Ladher, R.K. et al., 2000, Science 290:1965.