Fibroblast Growth Factor-21 (FGF-21) (UNQ3115/PRO10196)

Fibroblast Growth Factor-21 (FGF-21) belongs to the FGF family of heparin-binding polypeptides and is most closely related to FGF-23. Both proteins are synthesized as a precursor with hydrophobic signal peptides. FGF-21 is most highly expressed in the liver. Mature human FGF-21 shares 82%aa sequence homology with mature mouse FGF-21. \n\nApplications: \nSuitable for use in Flow Cytometry, Immunocytochemistry and ELISA. Other applications not tested.\n \nRecommended Dilution:\nFlow Cytometry: 25ug/ml; 10ul labels 1-5x10e5 HepG2 cells in a total reaction volume <200ul. For intercellular staining, cells must be fixed and permeabilized using PBS, 4% paraformaldehyde, 0.1% saponin.\nImmunocytochemistry: 10ug/ml; HepG2 human cells were fixed PBS, 4% paraformaldehyde for 20 min at RT and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-10, 1% BSA for 45 min at RT. Cells were incubated with F4212-93E overnight at 4C follwed by IgG (FITC) anti-mouse* at RT in the dark for 1 hour. Cells were washed with PBS, 0.1% BSA between each step.\nDirect ELISA: 0.5-1ug/ml; The detection limit is ~1ng/well.\nOptimal dilutions to be determined by the researcher.\n\n*Recommended Secondary Antibodies:\nI1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo\nI1904-65V IgG, (FITC) Pab RbxMo\nI1904-66A: IgG, (FITC) Pab Rb xMo\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.пїЅ

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SPECIFICATIONS

Catalog Number

F4212-93E

Size

100ug

Applications

ELISA, FC, ICC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2b

Additional Info

Recognizes human Fibroblast Growth Factor-21 (FGF-21). Does not crossreact with recombinant human FGF-5 or recombinant human FGF-9. Shows <10% crossreactivity with recombinant human FGF-3, 4, 6 and 10. Species Crossreactivity: mouse <10%.