Fibroblast Growth Factor-21 (FGF-21) belongs to the FGF family of heparin-binding polypeptides and is most closely related to FGF-23. Both proteins are synthesized as a precursor with hydrophobic signal peptides. FGF-21 is most highly expressed in the liver. Mature human FGF-21 shares 82%aa sequence homology with mature mouse FGF-21.
Applications:
Suitable for use in Flow Cytometry, Immunocytochemistry and ELISA. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 25ug/ml; 10ul labels 1-5x10e5 HepG2 cells in a total reaction volume <200ul. For intercellular staining, cells must be fixed and permeabilized using PBS, 4% paraformaldehyde, 0.1% saponin.
Immunocytochemistry: 10ug/ml; HepG2 human cells were fixed PBS, 4% paraformaldehyde for 20 min at RT and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-10, 1% BSA for 45 min at RT. Cells were incubated with F4212-93E overnight at 4C follwed by IgG (FITC) anti-mouse* at RT in the dark for 1 hour. Cells were washed with PBS, 0.1% BSA between each step.
Direct ELISA: 0.5-1ug/ml; The detection limit is ~1ng/well.
Optimal dilutions to be determined by the researcher.
*Recommended Secondary Antibodies:
I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo
I1904-65V IgG, (FITC) Pab RbxMo
I1904-66A: IgG, (FITC) Pab Rb xMo
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.�
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