FGF 4 was originally designated as hst, which was identified as a transforming gene in DNA samples from human stomach cancer, and shown to be secreted by both embryonic stem cells and embryonic carcinoma. Studies have shown that FGF 4 signals through binding to the FGF Receptors (FGFRs) 1c, 2c, 3c, and 4.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 0.25-1ug/ml
Western Blot: 0.1-0.2ug/ml
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:
Streptavidin APC
Streptavidin FITC
Streptavidin TRITC
Streptavidin RPE, RPE
Streptavidin HRP
Streptavidin Alk. Phos.
Storage and Stability:
Lyophilized powder may be stored at-20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with 0.5ml sterile PBS containing 0.1% BSA. Aliquot and store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.