Fibroblast Growth Factor, Acidic, Recombinant, Human (Acidic Fibroblast Growth Factor, aFGF, FGF-alpha, FGFA, Beta-endothelial Cell Growth Factor, ECGF-beta, ECGFB, ECGF, ECGFA, FGF1, GLIO703, Heparin-binding Growth Factor 1, HBGF1, HBGF-1)

Source:\nRecombinant corresponding to 155aa of human Fibroblast Growth Factor-acidic expressed in E.coli is a single, non-glycosylated,polypeptide chain.\n\nMolecular Weight:\n~17.5kD\n\nBiological Activity:\nThe ED50, calculated by the dose-dependent proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is less then 10ng/ml, corresponding to a specific activity of 10Units/mg.\n\nDimers and Aggregates:\n(same/less than)1% (SDS-PAGE)\n\nProtein Content:\nProtein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280nm. 2. Analysis by RP-HPLC, using a standard solution of FGF-acidic as a Reference Standard.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F4210-10J

Size

50ug

Form

Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to (same/more than)0.1mg/ml.

Purity

~95% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: (same/less than)0.1ng/ug (IEU/ug)

References

1. Immunolocalization of fibroblast growth factor-1 (FGF-1), its receptor (FGFR-1), and fibroblast-specific protein-1 (FSP-1) in inflammatory renal disease. Kidney Int 2005 Dec;68(6):2621-8. 2. Molecular Mechanism of Inhibition of Nonclassical FGF-1 Export(,). Biochemistry 2005 Nov 29;44(47):15472-15479. 3. An experimental and molecular-modeling study of the binding of linked sulfated tetracyclitols to FGF-1 and FGF-2. Chembiochem 2005 Oct;6(10):1882-90. 4. FGF-1 and S100A13 possibly contribute to angiogenesis in endometriosis. J Reprod Immunol 2005 Oct;67(1-2):87-101. 5. Effects of exogenous FGF-1 treatment on regeneration of the lens and the neural retina in the newt, Notophthalmus viridescens. J Exp Zoolog A Comp Exp Biol 2005 Oct 1;303(10):837-44. 6. Fibroblast Growth Factor (FGF)-1, FGF2, FGF7 and FGF Receptors are Uniformly Expressed in Trophoblast Giant Cells During Restricted Trophoblast Invasion in Cows. Placenta 2005 Aug 26;

Alternative Names

Fibroblast Growth Factor 1 (Acidic)