Fibroblast Growth Factor, Acidic, Recombinant, Human (FGFa)

Acidic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.\n\nRecombinant Human Fibroblast Growth Factor-acidic (FGF-1) is a single, non-glycosylated, polypeptide chain containing 141 amino acids and having a molecular mass of 15,967 Daltons.\n\nAmino Acid Sequence:\nMFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D\n\nBiological Activity:\nThe ED50, calculated by the dose-dependent proliferation of mouse BALB/c 3T3 cells is <1ng/ml.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

F4210-10A

Size

10ug

Form

Supplied as a lyophilized powder from PBS, pH 7.5. Reconstitute with sterile ddH2O to a concentration of 0.1-0.25mg/ml. Allow sample to sit for 5 minutes at 4C, spin to remove precipitant.

Purity

(same/more than) 97% as determined by: RP-HPLC and SDS-PAGE. Chromatographically purified.

References

1. Phosphorylation-regulated Nucleocytoplasmic Trafficking of Internalized FGF-1. Wiedlocha A, Nilsen T, Sorensen V, Mol Biol Cell 2004 Dec 1; 2. Incorporation of polymer microspheres within fibrin scaffolds for the controlled delivery of FGF-1. Royce SM, Askari M, J Biomater Sci Polym Ed 2004;15(10):1327-36 3. Role of AKT/PKB signaling in fibroblast growth factor-1 (FGF-1)-induced angiogenesis in the chicken chorioallantoic membrane (CAM). Forough R, Weylie B, Ambrus S, J Cell Biochem 2005 Jan 1;94(1):109-16 4. Testicular FGF-1 protein is involved in Sertoli cell-spermatid interaction in roe deer. Schon J, Blottner S, Gen Comp Endocrinol 2004 Nov;139(2):168-72 5. Design of fully active FGF-1 variants with increased stability. Zakrzewska M, Krowarsch D, Otlewski J, Protein Eng Des Sel 2004 Aug;17(8):603-11 6. Human FGF-1 gene transfer promotes the formation of collateral vessels and arterioles in ischemic muscles of hypercholesterolemic hamsters. Caron A, Michelet S, Sordello S, Schwartz B, J Gene Med 2004 Sep;6(9):1033-45