Fibroblast Growth Factor, Acidic, Recombinant, Human (FGFa, FGF1, aFGF, AFGF, Beta-endothelial cell growth factor, ECGF, ECGFA, ECGFB, ECGF-beta, FGFA, FGF-alpha, GLIO703, HBGF1, HBGF-1, Heparin-binding growth factor 1 precursor)

Single-chain polypeptide growth factor that plays a significant role in the process of wound healing and is a potent inducer of angiogenesis. It binds to heparin, which potentiates its biological activity and protects it from proteolysis. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages, and also has chemotactic and mitogenic activities. It was originally named acidic fibroblast growth factor based upon its chemical properties and to distinguish it from basic fibroblast growth factor. Other homologous FGF belonging to the same family are int-2 (FGF-3), FGF-5, FGF-6, K-FGF and KGF (keratinocyte growth factor =FGF-7 ). All factors are products of different genes, some of which are Oncogene products (FGF-3, FGF-4, FGF-5). \n\nRecombinant Human Fibroblast Growth Factor-acidic (FGF-1) produced in E.coli is a single, non-glycosylated, polypeptide chain containing 141 amino acids and having a molecular mass of 15,967 Daltons.\n\nAA Sequence:\nMFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D\n\nBiological Activity: Recombinant Human Fibroblast Growth Factor-alpha is fully biologically active when compared to standards. The ED50, calculated by the dose-dependent proliferation of mouse BALB/c 3T3 cells is <1.0 ng/ml.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, aliquot and store at -20 degrees C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F4210-08B

Size

10ug

Form

Supplied as a powder lyophilized from a solution containing PBS. Reconstitute with sterile dH2O to 0.1mg/ml, which can be further diluted into other aqueous solutions.

Purity

(same/more than) 97% as determined by RP-HPLC; Reducing and non-reducing SDS-PAGE. Endotoxin: (same/less than)0.1Eu/1ug (by LAL method)