FGF-acidic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-acidic is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. FGF-acidic has the ability to signal through all the FGF receptors. Recombinant murine FGF-acidic is a 15.9kD protein consisting of 141aa.
Source:
Recombinant protein corresponding to mouse FGF-acidic, expressed in E.coli.
Biological Activity:
Assay #1: Determined by the dose-dependent stimulation of thymidine uptake by 3T3 cells in the presence of heparin. The ED50 was found to be 0.5ng/ml corresponding to a specific activity of 2x10e6 units/mg.
Assay #2: Determined by a cell proliferation assay using balb/c 3T3 cells. The ED50 was found to be 0.2ng/ml in the presence of 10g/ml heparin, corresponding to a specific activity of 5x10e6 units/mg.
Endotoxin:
<0.1ng/ug (1EU/ug)
AA Sequence:
MFNLPLGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SAGEVYIKGT ETGQYLAMDT EGLLYGSQTP NEECLFLERL EENHYNTYTS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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