Fibroblast Growth Factor, Acidic, Recombinant, Mouse (FGFa, FGF1, aFGF, AFGF, Beta-endothelial cell growth factor, ECGF, ECGFA, ECGFB, ECGF-beta, FGFA, FGF-alpha, GLIO703, HBGF1, HBGF-1, Heparin-binding growth factor 1 precursor)

The fibroblast growth factors play a central role in prenatal development and growth and regeneration of cells during the proliferation and differentiation stages. M-FGF-acidic is non-glycosylated heparin binding protein comprised of 141 amino acids.\n\nBiological Activity: Recombinant Mouse FGF-alpha is fully biologically active when compared to standards. The ED50, calculated by the dose-dependent proliferation of mouse BALB/c 3T3 cells, is 0.04ng/ml.\n\nAA Sequence:\nMFNLPLGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SAGEVYIKGT ETGQYLAMDT EGLLYGSQTP NEECLFLERL EENHYNTYTS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, aliquot and store at -20 degrees C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F4210-02B

Size

10ug

Form

Supplied as a powder lyophilized from a solution containing PBS. Reconstitute with sterile dH2O to 0.1-0.5mg/ml, which can be further diluted into other aqueous solutions.

Purity

(same/more than) 98% as determined by RP-HPLC; Reducing and non-reducing SDS-PAGE. Endotoxin: (same/less than)0.1Eu/1ug (by LAL method)