Fibroblast Growth Factor, Basic, Human (FGFb) BioAssay(TM) ELISA Kit

Human bFGF ELISA Kit is for the quantitative determination of human basic fibroblast growth factor (bFGF) concentrations in serum, plasma, cell culture supernatant, urine, and other biological fluids.\n\nThe bFGF ELISA is a 2.5 hour solid phase immunoassay readily applicable to measure bFGF levels in serum, plasma, cell culture supernatant, urine, and other biological fluids in the range of 0 to 8000 pg/mL. It showed no crossreactivity with other cytokines tested. This assay recognizes natural human bFGF, recombinant bFGF. This bFGF ELISA is expected to be effectively used for further investigations into the relationship between bFGF and the various conditions mentioned.\n\nSensitivity: 78pg/mL.\n\nRange: 78-8000pg/mL\n\nPrinciple:\nThe bFGF enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal specific for bFGF. Standards or samples are then added to the appropriate microtiter plate wells and incubated. bFGF if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound bFGF and other components of sample. In order to quantitative the amount of bFGF present in the sample, a standardized preparation of horseradish peroxidase HRP)-conjugated polyclonal antibody specific for bFGF is added to each well to "sandwich" the bFGF immobilised during the first incubation. The microtiter plate then undergoes a second incubation. The wells are thoroughly washed to remove all unbound HRP-conjugated antibodies and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. \nThe enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain bFGF and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured by spectrophotometer at a wavelength of 450nm

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