FGF-basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-basic is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b, 1c, 2c, 3c and 4. Recombinant human FGF-basic is a 17.2kD protein consisting of 154aa.
Source:
Recombinant protein corresponding to human FGF-basic, expressed in E. coli.
Biological Activity:
Assay #1: Determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors. The ED50 was found to be 0.5ng/ml, corresponding to a specific activity of 2x10e6units/mg.
Assay #2: Determined by a cell proliferation assay using balb/c 3T3 cells. The ED50 was found to be 0.1ng/ml, corresponding to a specific activity of 1x10e7units/mg.
Endotoxin:
<0.1ng/ug (1EU/ug)
AA Sequence:
AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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