Fibroblast Growth Factor, Basic, Recombinant, Mouse (FGFb)

Mouse FGF basic is a single-chain polypeptide growth factor that plays a significant role in the process of wound healing and is a potent inducer of angiogenesis. Several different forms of the protein exist ranging from 18-24kD in size due to the use of alternative start sites within the fgf-2 gene. It has a 55 percent amino acid residue identity to FGF-1 and has potent heparin-binding activity. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages. It was originally named basic fibroblast growth factor based upon its chemical properties and to distinguish it from acidic fibroblast growth factor.Other homologous FGF belonging to the same family are int-2 (FGF-3), FGF-5, FGF-6, K-FGF and KGF (keratinocyte growth factor =FGF-7 ). All factors are products of different genes, some of which are Oncogene products (FGF-3, FGF-4, FGF-5). \n\nBiological Activity: The ED50, calculated by the dose-dependent proliferation of BALB/3T3 cells is <0.5ng/ml.\n\nAA Sequence:\nMPALPEDGGA AFPPGHFKDP KRLYCKNGGF FLRIHPDGRV DGVREKSDPH VKLQLQAEER GVVSIKGVCA NRYLAMKEDG RLLASKCVTE ECFFFERLES NNYNTYRSRK YSSWYVALKR TGQYKLGSKT GPGQKAILFL PMSAKS \n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, aliquot and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F4210-20G

Size

10ug

Form

Supplied as a lyophilized powder. Reconstitute with sterile dH2O PBS to 0.1-1mg/ml, which can be further diluted into other aqueous solutions.

Purity

(same/more than) 95% as determined by RP-HPLC; Reducing and non-reducing SDS-PAGE.

References

1. The fibroblast growth factor-2 antisense gene inhibits nuclear accumulation of FGF-2 and delays cell cycle progression in C6 glioma cells. Mol Cell Endocrinol 2007 Jan 21;\n2. Amelioration of diabetes in mice after single-donor islet transplantation using the controlled release of gelatinized FGF-2. Cell Transplant 2006;15(10):939-44\n3. Frequent immunoexpression of TGF-beta1, FGF-2 and BMP-2 in fibroblast-like cells in osteofibrous dysplasia. Oncol Rep 2007 Mar;17(3):531-5\n4. Fibroblast growth factor (FGF)-2 and FGF receptor 3 are required for the development of the substantia nigra, and FGF-2 plays a crucial role for the rescue of dopaminergic neurons after 6-hydroxydopamine lesion. J Neurosci 2007 Jan 17;27(3):459-71\n5. Ovine endometrial expression of fibroblast growth factor (FGF) 2 and conceptus expression of FGF receptors during early pregnancy. Domest Anim Endocrinol 2007 Jan 3;\n6. Retinal and olfactory bulb precursor cells show distinct responses to FGF-2 and laminin.\nCell Biol Int 2006 Dec 5; murine mouse basic fgf-b FGF-basic m fibroblast growth factor FGF FGF-B M-FGF-b 200-12