Fibroblast Growth Factor, Basic, Recombinant, Mouse (FGFb)

Source: \nRecombinant corresponding to mouse Fibroblast Growth Factor, Basic expressed in E. coli.\n\nMolecular Weight: \n~17kD\n\nActivity:\nED50 range =0.1-1.0 ng/ml, determined by the dose dependent proliferatio of BALB/3T3 cells. The optimal concentration for each specific application should be determined by an initial dose response assay.\n\nEndotoxin: (same/less than)0.1ng/ug\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F4210-20M

Size

10ug

Form

Supplied as a lyophilized powder. BSA free. Reconstitute with 0.1-0.5mg/ml sterile dH2O.

Purity

~95% (SDS-PAGE)

References

1. Herbert, J.M., C. Basilico, M. Goldfarb, O. Haub and G.R. Martin (1990) Isolation of cDNAs encoding four mouse FGF family members and characterization of their expression patterns during embryogenesis. Dev. Biol. 138:454-463.\n\n2. Seddon, A., M. Decker, T. Muller, D. Armellino, I. Kovesdi, Y. Gluzman, and P. Bohlen (1991) Structure/activity relationships in basic FGF. Ann. N.Y. Acad. Sci. 638:98-105.\n\n3. Kitchens, D.L., E. Snyder, and D. Gottlieb (1994) FGF and EGF are mitogens for immortalized neural progenitors. J. Neurobiol. 25:797-807.\n\n4. Temple, S., and X. Qian (1995) bFGF, neurotrophins, and the control of cortical neurogenesis. Neuron 15:249-252.\n\n5. Ding, I., K. Huang, M.L. Snyder, J. Cook, L. Zhang, N. Wersto, and P. Okunieff (1996) Tumor growth and tumor radiosensitivity in mice given myeloprotective doses of fibroblast growth factors. J. Nat